Open AccessPolydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell
Author(s) -
Sijia Chen,
Jing Tao,
Fengyun Zhong,
Yang Jiao,
Jiao Xu,
Qiang Shen,
Haichao Wang,
Saijun Fan,
Yusong Zhang
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0176501
Subject(s) - cell cycle , western blot , cell growth , apoptosis , phosphorylation , kinase , creb , microbiology and biotechnology , cell cycle checkpoint , flow cytometry , biology , chemistry , cancer research , biochemistry , transcription factor , gene
Polydatin (PD), a component isolated from Polygonum cuspidatum , has a number of biological functions. However, the antitumor activity of PD has been poorly investigated. In this study, the effect of PD on cell proliferation was evaluated by thiazolyl blue tetrazolium bromide assay. Cell cycle distribution and apoptosis were investigated by flow cytometry. The phosphorylation levels of panel of phosphor-kinases were detected by human phospho-kinase arrays. The expression of several proteins associated with cell cycle and apoptosis were analyzed by Western blot analysis. Results showed that PD effectively inhibited the growth of MDA-MB-231 and MCF-7 breast cancer cell lines. Cell cycle analysis demonstrated that PD induced S-phase cell cycle arrest. Human phosphor-kinase arrays showed that the phosphorylation level of cAMP response element-bingding proteins(Creb) was down-regulated, and the results were further confirmed by Western blot analysis. Western blot analysis showed that the expression of protein of cyclin D1 decreased in a time- and dose- dependent manner. Results suggest that PD is a potential therapeutic natural compound.