Open AccessMetabolic crosstalk between membrane and storage lipids facilitates heat stress management in Schizosaccharomyces pombe
Author(s) -
Mária Péter,
Attila Glatz,
Péter Gudmann,
Imre Gombos,
Zsolt Török,
Ibolya Horváth,
László Vı́gh,
Gábor Balogh
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0173739
Subject(s) - schizosaccharomyces pombe , lipidome , schizosaccharomyces , membrane , yeast , membrane lipids , crosstalk , microbiology and biotechnology , chemistry , biochemistry , lipidomics , lipid metabolism , biology , saccharomyces cerevisiae , physics , optics
Cell membranes actively participate in stress sensing and signalling. Here we present the first in-depth lipidomic analysis to characterize alterations in the fission yeast Schizosaccharomyces pombe in response to mild heat stress (HS). The lipidome was assessed by a simple one-step methanolic extraction. Genetic manipulations that altered triglyceride (TG) content in the absence or presence of HS gave rise to distinct lipidomic fingerprints for S . pombe . Cells unable to produce TG demonstrated long-lasting growth arrest and enhanced signalling lipid generation. Our results reveal that metabolic crosstalk between membrane and storage lipids facilitates homeostatic maintenance of the membrane physical/chemical state that resists negative effects on cell growth and viability in response to HS. We propose a novel stress adaptation mechanism in which heat-induced TG synthesis contributes to membrane rigidization by accommodating unsaturated fatty acids of structural lipids, enabling their replacement by newly synthesized saturated fatty acids.