Open AccessHg tolerance and biouptake of an isolated pigmentation yeast Rhodotorula mucilaginosa
Author(s) -
Bing Liu,
Chaogang Wang,
Danxia Liu,
Ning He,
Xu Deng
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0172984
Subject(s) - yeast , rhodotorula , bioavailability , chemistry , aqueous solution , phosphate , biochemistry , x ray photoelectron spectroscopy , nuclear chemistry , biology , organic chemistry , pharmacology , physics , nuclear magnetic resonance
A pigmented yeast R1 with strong tolerance to Hg 2+ was isolated. Phylogenetic identification based on the analysis of 26S rDNA and ITS revealed R1 is a Rhodotorula mucilaginosa species. R1 was able to grow in the presence of 80 mg/L Hg 2+ , but the lag phase was much prolonged compared to its growth in the absence of Hg 2+ . The maximum Hg 2+ binding capacity of R1 was 69.9 mg/g, and dead cells could bind 15% more Hg 2+ than living cells. Presence of organic substances drastically reduced bioavailability of Hg 2+ and subsequently decreased Hg 2+ removal ratio from aqueous solution, but this adverse effect could be remarkably alleviated by the simultaneous process of cell propagation and Hg 2+ biouptake with actively growing R1. Furthermore, among the functional groups involved in Hg 2+ binding, carboxyl group contributed the most, followed by amino & hydroxyl group and phosphate group. XPS analysis disclosed the mercury species bound on yeast cells was HgCl 2 rather than HgO or Hg 0 .