Open AccessArachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release
Author(s) -
Joaquín Paillamanque,
Ana A. SánchezTusie,
Emerson M. Carmona,
Claudia L. Treviño,
Carolina Ortiz Sandoval,
Francisco Nualart,
Nelson Osses,
Juan G. Reyes
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0172128
Subject(s) - intracellular , microbiology and biotechnology , arachidonic acid , ryanodine receptor , biology , sertoli cell , chemistry , biochemistry , spermatogenesis , endocrinology , enzyme
Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca 2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca 2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca 2+ using fluorescent Ca 2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca 2+ stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca 2+ release from intracellular Ca 2+ stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.