Open AccessInvestigation of the fungal community structures of imported wheat using high-throughput sequencing technology
Author(s) -
Yaqian Shi,
Cheng Yinghui,
Ying Wang,
Guiming Zhang,
Ruifang Gao,
Caiyu Xiang,
Juan Feng,
Dingfeng Lou,
Ying Li
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0171894
Subject(s) - biology , internal transcribed spacer , dothideomycetes , illumina dye sequencing , unifrac , botany , dna sequencing , ascomycota , plant use of endophytic fungi in defense , metagenomics , phylum , ribosomal rna , 16s ribosomal rna , bacteria , genetics , gene
This study introduced the application of high-throughput sequencing techniques to the investigation of microbial diversity in the field of plant quarantine. It examined the microbial diversity of wheat imported into China, and established a bioinformatics database of wheat pathogens based on high-throughput sequencing results. This study analyzed the nuclear ribosomal internal transcribed spacer (ITS) region of fungi through Illumina Miseq sequencing to investigate the fungal communities of both seeds and sieve-through. A total of 758,129 fungal ITS sequences were obtained from ten samples collected from five batches of wheat imported from the USA. These sequences were classified into 2 different phyla, 15 classes, 33 orders, 41 families, or 78 genera, suggesting a high fungal diversity across samples. Apairwise analysis revealed that the diversity of the fungal community in the sieve-through is significantly higher than those in the seeds. Taxonomic analysis showed that at the class level, Dothideomycetes dominated in the seeds and Sordariomycetes dominated in the sieve-through. In all, this study revealed the fungal community composition in the seeds and sieve-through of the wheat, and identified key differences in the fungal community between the seeds and sieve-through.