Open AccessPurification, characterization, and antitumor activity of a novel glucan from the fruiting bodies of Coriolus Versicolor
Author(s) -
Annoor Awadasseid,
Jie Hou,
Yaser Gamallat,
Xiaona Shang,
Kuugbee D. Eugene,
Ahmed Musa Hago,
Djibril Bamba,
Abdo Meyiah,
Gift Chiwala,
Yi Xin
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0171270
Subject(s) - in vivo , spleen , in vitro , chemistry , glucan , sarcoma , body weight , pharmacology , biochemistry , immunology , biology , medicine , pathology , endocrinology , microbiology and biotechnology
Cancer is one of the most common causes of deaths worldwide. Herein, we report an efficient natural anticancer glucan (CVG) extracted from Coriolus Versicolar (CV). CVG was extracted by the hot water extraction method followed by ethanol precipitation and purified using gas exclusion chromatography. Structural analysis revealed that CVG has a linear α-glucan chain composed of only (1→ 6)-α-D-Glc p . The antitumor activity of CVG on Sarcoma-180 cells was investigated in vitro and in vivo . Mice were treated with three doses of CVG (40, 100, 200 mg/kg body weight) for 9 days. Tumor weight, relative spleen, thymus weight, and lymphocyte proliferation were studied. A significant increase ( P< 0 . 01 ) in relative spleen and thymus weight and a decrease ( P< 0 . 01 ) in tumor weight at the doses of 100 and 200 mg/kg were observed. The results obtained demonstrate CVG has antitumor activity towards Sarcoma-180 cells by its immunomodulation activity.