Open AccessGenomic confirmation of vancomycin-resistant Enterococcus transmission from deceased donor to liver transplant recipient
Author(s) -
Ali Kashif Bashir,
Oliver Attie,
Mitchell J. Sullivan,
Robert Sebra,
Kavindra V. Singh,
Deena R. Altman,
Theodore R. Pak,
Jayeeta Dutta,
Kieran I. Chacko,
Elizabeth Webster,
Martha Lewis,
Camille Hamula,
Kristin W. Delli Carpini,
Barbara E. Murray,
Andrew Kasarskis,
Harm van Bakel,
Shirish Huprikar
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0170449
Subject(s) - multilocus sequence typing , genome , biology , typing , whole genome sequencing , transmission (telecommunications) , pathogen , genetics , microbiology and biotechnology , gene , genotype , electrical engineering , engineering
In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.