Open AccessThe Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages
Author(s) -
Meret Cepero Malo,
Anne-Laure Duchemin,
Luca Guglielmi,
Eva Patzel,
Saadettin Sel,
Gerd U. Auffarth,
Michael Carl,
Lucia Poggi
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0170356
Subject(s) - green fluorescent protein , microbiology and biotechnology , biology , zebrafish , transgene , embryonic stem cell , live cell imaging , mitosis , stem cell , genetics , cell , gene
Monitoring cycling behaviours of stem and somatic cells in the living animal is a powerful tool to better understand tissue development and homeostasis. The tg( anillin : anillin-eGFP ) transgenic line carries the full-length zebrafish F-actin binding protein Anillin fused to eGFP from a bacterial artificial chromosome (BAC) containing Anillin cis-regulatory sequences. Here we report the suitability of the Anillin-eGFP reporter as a direct indicator of cycling cells in the late embryonic and post-embryonic retina. We show that combining the anillin : anillin-eGFP with other transgenes such as ptf1a : dsRed and atoh7 : gap-RFP allows obtaining spatial and temporal resolution of the mitotic potentials of specific retinal cell populations. This is exemplified by the analysis of the origin of the previously reported apically and non-apically dividing late committed precursors of the photoreceptor and horizontal cell layers.