Open AccessReduction of Aflatoxin B1 Toxicity by Lactobacillus plantarum C88: A Potential Probiotic Strain Isolated from Chinese Traditional Fermented Food “Tofu”
Author(s) -
Li Huang,
Cuicui Duan,
Yujuan Zhao,
Lei Gao,
Chunhua Niu,
Jianling Xu,
Shengyu Li
Publication year - 2017
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0170109
Subject(s) - lactobacillus plantarum , antioxidant , aflatoxin , glutathione , oxidative stress , probiotic , chemistry , toxicity , biochemistry , biology , food science , enzyme , lactic acid , bacteria , genetics , organic chemistry
In this study, we investigated the potential of Lactobacillus plantarum isolated from Chinese traditional fermented foods to reduce the toxicity of aflatoxin B 1 (AFB 1 ), and its subsequent detoxification mechanism. Among all the investigated L . plantarum strains, L . plantarum C88 showed the strongest AFB 1 binding capacity in vitro, and was orally administered to mice with liver oxidative damage induced by AFB 1 . In the therapy groups, the mice that received L . plantarum C88, especially heat-killed L . plantarum C88, after a single dose of AFB 1 exposure, showed an increase in unabsorbed AFB 1 in the feces. Moreover, the effects of L . plantarum C88 on the enzymes and non-enzymes antioxidant abilities in serum and liver, histological alterations of liver were assayed. The results indicated that compared to the control group, L . plantarum C88 alone administration induced significant increase of antioxidant capacity, but did not induce any significant changes in the histological picture. Compared to the mice that received AFB 1 only, L . plantarum C88 treatment could weaken oxidative stress by enhancing the activity of antioxidant enzymes and elevating the expression of Glutathione S-transferase (GST) A3 through Nuclear factor erythroid (derived factor 2) related factor 2 (Nrf2) pathway. Furthermore, cytochrome P450 (CYP 450) 1A2 and CYP 3A4 expression was inhibited by L . plantarum C88, and urinary aflatoxin B 1 - N 7 -guanine (AFB- N 7 -guanine), a AFB 1 metabolite formed by CYP 1A2 and CYP 3A4, was significantly reduced by the presence of viable L . plantarum C88. Meanwhile, the significant improvements were showed in histological pictures of the liver tissues in mice orally administered with viable L . plantarum C88. Collectively, L . plantarum C88 may alleviate AFB 1 toxicity by increasing fecal AFB 1 excretion, reversing deficits in antioxidant defense systems and regulating the metabolism of AFB 1 .