Open AccessForsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice
Author(s) -
YoonKyoung Sung,
Taesook Yoon,
Seol Jang,
Ho Kyoung Kim
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0167687
Subject(s) - atopic dermatitis , chemokine , house dust mite , histamine , immunology , allergy , tumor necrosis factor alpha , proinflammatory cytokine , immunoglobulin e , in vivo , pharmacology , human skin , inflammation , medicine , allergen , chemistry , biology , antibody , genetics , microbiology and biotechnology
Forsythia suspensa ( F . suspensa ) is a traditional medicine for treatment of inflammation. In this study, we evaluated the therapeutic effects of an ethanol extract from F . suspensa fruits on atopic dermatitis both in vivo and in vitro . We investigated the inhibitory effects of F . suspensa extract on the development of atopic dermatitis-like skin lesions in an NC/Nga mouse model exposed to Dermatophagoides farinae crude extract. Topical application of F . suspensa extract to the mice attenuated the atopic dermatitis symptoms, including increased dermatitis severity score, ear thickness, infiltration of inflammatory cells in the skin lesions, serum levels of IgE, TNF-α, and histamine, and expression of chemokines, cytokines, and adhesion molecules in ear tissue. In addition, F . suspensa extract inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. High-performance liquid chromatography analysis of FSE revealed the presence of four chemical constituents (forsythiaside, phillyrin, pinoresinol, and phylligenin). These compounds inhibited the production of chemokines in TNF-α/IFN-γ-activated human keratinocytes. These results suggest that the F . suspensa might be a useful candidate for treating allergic skin inflammatory disorders.