Open AccessKluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
Author(s) -
Ida M. Smith,
Adam Baker,
Jeffrey E. Christensen,
Teun Boekhout,
Hanne Frøkiær,
Nils Arneborg,
Lene Jespersen
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0167410
Subject(s) - saccharomyces boulardii , immune system , kluyveromyces marxianus , biology , t cell , cytokine , dendritic cell , microbiology and biotechnology , secretion , foxp3 , probiotic , immunology , yeast , saccharomyces cerevisiae , biochemistry , bacteria , genetics
Interactions between members of the intestinal microbiota and the mucosal immune system can significantly impact human health, and in this context, fungi and food-related yeasts are known to influence intestinal inflammation through direct interactions with specialized immune cells in vivo . The aim of the present study was to characterize the immune modulating properties of the food-related yeast Kluyveromyces marxianus in terms of adaptive immune responses indicating inflammation versus tolerance and to explore the mechanisms behind the observed responses. Benchmarking against a Saccharomyces boulardii strain with probiotic effects documented in clinical trials, we evaluated the ability of K . marxianus to modulate human dendritic cell (DC) function in vitro . Further, we assessed yeast induced DC modulation of naive T cells toward effector responses dominated by secretion of IFNγ and IL-17 versus induction of a T reg response characterized by robust IL-10 secretion. In addition, we blocked relevant DC surface receptors and investigated the stimulating properties of β-glucan containing yeast cell wall extracts. K . marxianus and S . boulardii induced distinct levels of DC cytokine secretion, primarily driven by Dectin-1 recognition of β-glucan components in their cell walls. Upon co-incubation of yeast exposed DCs and naive T cells, S . boulardii induced a potent IFNγ response indicating T H 1 mobilization. In contrast, K . marxianus induced a response dominated by Foxp3 + T reg cells, a characteristic that may benefit human health in conditions characterized by excessive inflammation and positions K . marxianus as a strong candidate for further development as a novel yeast probiotic.