Open AccessRegulation and Maintenance of an Adoptive T-Cell Dependent Memory B Cell Pool
Author(s) -
Marie Anson,
Inês F. Amado,
Marie-Pierre Mailhé,
Emmanuel Donnadieu,
Sylvie Garcia,
François Huetz,
António A. Freitas
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0167003
Subject(s) - adoptive cell transfer , germinal center , memory b cell , b 1 cell , naive b cell , b cell , biology , priming (agriculture) , antigen , immunology , immune system , population , antibody , t cell , microbiology and biotechnology , antigen presenting cell , medicine , botany , germination , environmental health
We investigated the ability of monoclonal B cells to restore primary and secondary T-cell dependent antibody responses in adoptive immune-deficient hosts. Priming induced B cell activation and expansion, AID expression, antibody production and the generation of IgM + IgG - and IgM - IgG + antigen-experienced B-cell subsets that persisted in the lymphopenic environment by cell division. Upon secondary transfer and recall the IgM - IgG + cells responded by the production of antigen-specific IgG while the IgM + memory cells secreted mainly IgM and little IgG, but generated new B cells expressing germinal center markers. The recall responses were more efficient if the antigenic boost was delayed suggesting that a period of adaptation is necessary before the transferred cells are able to respond. Overall these findings indicate that reconstitution of a functional and complete memory pool requires transfer of all different antigen-experienced B cell subsets. We also found that the size of the memory B cell pool did not rely on the number of the responding naïve B cells, suggesting autonomous homeostatic controls for naïve and memory B cells. By reconstituting a stable memory B cell pool in immune-deficient hosts using a monoclonal high-affinity B cell population we demonstrate the potential value of B cell adoptive immunotherapy.