Open AccessmiR-125b Enhances IL-8 Production in Early-Onset Severe Preeclampsia by Targeting Sphingosine-1-Phosphate Lyase 1
Author(s) -
Weiwei Yang,
Anning Wang,
Chunling Zhao,
Qinghua Li,
Zhifang Pan,
Xiao Han,
Cuijuan Zhang,
Guohui Wang,
Chao Ji,
Guili Wang,
Guanqing Jia,
Jiyu Ju,
Wei Gao,
Wei Yu,
Xiaoying Liu,
Xi Chen,
Weiguo Feng,
Zhiqin Gao,
Jie Li,
Chune Ren
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0166940
Subject(s) - preeclampsia , sphingosine 1 phosphate , pathogenesis , luciferase , inflammation , downregulation and upregulation , endocrinology , medicine , andrology , interleukin 6 , sphingosine , chemistry , immunology , biology , pregnancy , biochemistry , transfection , gene , receptor , genetics
Preeclampsia (PE) is one of the leading causes of maternal and perinatal mortality and morbidity. One of the main hallmarks observed in PE is impaired inflammation state. In the current study, we found that miR-125b was deregulated in placental tissues and plasma derived from PE patients, which suggest a potential association between this miRNA and the pathogenesis of PE. Overexpression of miR-125b significantly reduced SGPL1 expression, and luciferase assays confirmed that SGPL1 is a direct target of miR-125b. We also found that miR-125b enhanced IL-8 production by directly targeting sphingosine-1-phosphate lyase 1 (SGPL1), and this effect could be reversed by SGPL1 overexpression. In placentas derived from PE patients, a negative correlation of miR-125b and SGPL1 was observed, and IL-8 was validated to be increased in the circulation of PE patients. Our data demonstrated a critical role of miR-125b in IL-8 production and the development of PE.