Open AccessStromal Cell-Derived Factor 1 Polymorphism in Retinal Vein Occlusion
Author(s) -
Andrea Szigeti,
Mónika Ecsedy,
Miklós Schneider,
Lillà Lionetti,
Balázs Lesch,
Zoltán Zsolt Nagy,
Andrea Fekete,
Zsuzsa Récsán
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0166544
Subject(s) - genotype , occlusion , allele , medicine , gastroenterology , retinal vein , biology , genetics , gene
Background Stromal cell-derived factor 1 ( SDF1 ) has crucial role in the regulation of angiogenesis and ocular neovascularisation (NV). The purpose of this study was to evaluate the association between SDF1 -3’G(801)A polymorphism and NV complications of retinal vein occlusion (RVO). Methods 130 patients with RVO (median age: 69.0, range 35–93 years; male/female– 58/72; 55 patients had central RVO, 75 patients had branch RVO) were enrolled in this study. In the RVO group, 40 (30.8%) patients were diagnosed with NV complications of RVO and 90 (69.2%) patients without NVs. The median follow up period was 40.3 months (range: 18–57 months). The SDF1 -3’G(801)A polymorphism was detected by PCR-RFLP. Allelic prevalence was related to reference values obtained in the control group consisted of 125 randomly selected, age and gender matched, unrelated volunteers (median age: 68.0, range 36–95 years; male/female– 53/72). Statistical analysis of the allele and genotype differences between groups (RVO patients vs controls; RVO patients with NV vs RVO patients without NV) was determined by chi-squared test. P value of <0.05 was considered statistically significant. Results Hardy-Weinberg criteria was fulfilled in all groups. The SDF1- 3’G(801)A allele and genotype frequencies of RVO patients were similar to controls ( SDF1 -3’A allele: 22.3% vs 20.8%; SDF1 -3’(801)AA: 5.4% vs 4.8%, SDF1 -3’(801)GG: 60.8% vs 63.2%). The frequency of SDF1 -3’(801)AA and SDF1 -3’(801)GA genotypes, as well as the SDF1- 3’(801)A allele frequency were higher in RVO patients with NV versus in patients without NV complication ( SDF1 -3’(801)AA+AG genotypes: 57.5% vs 31.1%, p = 0.008; SDF1 -3’(801)A allele: 35.0% vs 16.7%, p = 0.002) or versus controls ( SDF1 -3’(801)AA+AG genotypes 57.5% vs 36.8%, p = 0.021; SDF1 -3’(801)A allele: 35.0% vs 20.8% p = 0.01). Carrying of SDF1 -3’(801)A allele increased the risk of neovascularisation complications of RVO by 2.69 (OR, 95% CI = 1.47–4.93). Conclusion These findings suggest that carrying SDF1 -3’(801)A allele plays a role in the development of neovascular complications in retinal vein occlusion.