Open AccessTranscriptome Analysis of the Midgut of the Chinese Oak Silkworm Antheraea pernyi Infected with Antheraea pernyi Nucleopolyhedrovirus
Author(s) -
Xisheng Li,
Guobao Wang,
Yu Sun,
Wei Liu,
Yingzi He,
Fengcheng Wang,
Yiren Jiang,
Qin Li
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0165959
Subject(s) - antheraea pernyi , transcriptome , midgut , biology , kegg , defensin , innate immune system , gene , genetics , microbiology and biotechnology , gene expression , botany , immune system , larva
The Antheraea pernyi nucleopolyhedrovirus (ApNPV) is an exclusive pathogen of A . pernyi . The intense interactions between ApNPV and A . pernyi cause a series of physiological and pathological changes to A . pernyi . However, no detailed report exists regarding the molecular mechanisms underlying the interactions between ApNPV and A . pernyi . In this study, four cDNA libraries of the A . pernyi midgut, including two ApNPV-infected groups and two control groups, were constructed for transcriptomic analysis to provide new clues regarding the molecular mechanisms that underlie these interactions. The transcriptome of the A . pernyi midgut was de novo assembled using the Trinity platform because of the lack of a genome resource for A . pernyi . Compared with the controls, a total of 5,172 differentially expressed genes (DEGs) were identified, including 2,183 up-regulated and 2,989 down-regulated candidates, of which 2,965 and 911 DEGs were classified into different GO categories and KEGG pathways, respectively. The DEGs involved in A . pernyi innate immunity were classified into several categories, including heat-shock proteins, apoptosis-related proteins, serpins, serine proteases and cytochrome P450s. Our results suggested that these genes were related to the immune response of the A . pernyi midgut to ApNPV infection via their essential roles in regulating a variety of physiological processes. Our results may serve as a basis for future research not only on the molecular mechanisms of ApNPV invasion but also on the anti-ApNPV mechanism of A . pernyi .