Open AccessChanges to the Aqueous Humor Proteome during Glaucoma
Author(s) -
Martha Kaeslin,
Hanspeter E. Killer,
Cyril Adrian Fuhrer,
Nauke Zeleny,
Andreas Huber,
Albert Neutzner
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0165314
Subject(s) - proteome , aqueous humor , glaucoma , downregulation and upregulation , proteomics , ophthalmology , metabolome , aqueous solution , medicine , chemistry , biochemistry , biology , bioinformatics , metabolomics , gene
Purpose To investigate the aqueous humor proteome in patients with glaucoma and a control group. Method Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed. Results Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor. Of the 87 proteins, 34 were significantly upregulated, whereas 53 proteins were downregulated in the aqueous humor from glaucoma patients compared to controls. Differentially expressed proteins were found to be involved in cholesterol-related, inflammatory, metabolic, antioxidant as well as proteolysis-related processes. Conclusion Glaucoma leads to profound changes to the aqueous humor proteome consistent with an altered metabolic state, an inflammatory response and impaired antioxidant defense.