Open AccessGlobal Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin
Author(s) -
Can Chen,
Junfeng Pan,
Xiaobing Yang,
Chenghao Guo,
Wei Ding,
Meiru Si,
Yi Zhang,
Xihui Shen,
Yao Wang
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0164955
Subject(s) - corynebacterium glutamicum , vanillin , lignocellulosic biomass , chemistry , transcriptome , saccharomyces cerevisiae , metabolic engineering , biochemistry , biomass (ecology) , biofuel , fermentation , food science , yeast , biology , gene , microbiology and biotechnology , gene expression , agronomy
Lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. Vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. To assess the response of Corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. The transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related to the stress response, ribosome/translation, protein secretion, and the cell envelope. Moreover, deletion of the sigH or msrA gene in C . glutamicum resulted in a decrease in cell viability under vanillin stress. These insights will promote further engineering of model industrial strains, with enhanced tolerance or degradation ability to vanillin to enable suitable production of biofuels and bio-based chemicals from lignocellulosic biomass.