Open AccessConstruction and Rescue of a Molecular Clone of Deformed Wing Virus (DWV)
Author(s) -
Benjamin Lamp,
Angelika Url,
Kerstin Seitz,
Jürgen Eichhorn,
Christiane Riedel,
Leonie Sinn,
Stanislav Indik,
Hemma Köglberger,
Till Rümenapf
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0164639
Subject(s) - deformed wing virus , biology , virology , virus , tissue tropism , tropism , genetics , honey bee , varroa destructor , zoology
European honey bees are highly important in crop pollination, increasing the value of global agricultural production by billions of dollars. Current knowledge about virulence and pathogenicity of Deformed wing virus (DWV), a major factor in honey bee colony mortality, is limited. With this study, we close the gap between field research and laboratory investigations by establishing a complete in vitro model for DWV pathogenesis. Infectious DWV was rescued from a molecular clone of a DWV-A genome that induces DWV symptoms such as crippled wings and discoloration. The expression of DWV proteins, production of infectious virus progeny, and DWV host cell tropism could be confirmed using newly generated anti-DWV monoclonal antibodies. The recombinant RNA fulfills Koch’s postulates circumventing the need of virus isolation and propagation of pure virus cultures. In conclusion, we describe the development and application of a reverse genetics system for the study of DWV pathogenesis.