Open AccessEffects of Isaria fumosorosea on TYLCV (Tomato Yellow Leaf Curl Virus) Accumulation and Transmitting Capacity of Bemisia tabaci
Author(s) -
Bowen Zhang,
Chunhua Zhang,
Qiongbo Hu
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0164356
Subject(s) - tomato yellow leaf curl virus , nymph , biology , instar , entomopathogenic fungus , transmission (telecommunications) , leaf curl , whitefly , virology , horticulture , fungus , plant virus , botany , virus , veterinary medicine , biological pest control , larva , medicine , beauveria bassiana , electrical engineering , engineering
Tomato yellow leaf curl virus ( TYLCV ) is transmitted by the Bemisia tabaci pest Middle East-Asia Minor 1 (MEAM1) in China. Isaria fumosorosea is a fungal pathogen of B . tabaci . However, the effects of fungal infection on TYLCV expression and transmission by MEAM1 are unclear. In this study, potted tomatoes containing second instar nymphs of MEAM1 were treated with I . fumosorosea IfB01 strain and the relationship between fungal infection in MEAM1 and its TYLCV transmission capacity was investigated. The results indicated that a significantly (p < 0.05) decreased incidence of transmission of TYLCV -infected plants (ITYPs) transmitted by second instar nymphs of MEAM1 infected with fungus. Further, we found a negative correlation between fungal conidial concentrations and eclosion rates of MEAM1, and a positive correlation between ITYPs and eclosion. In addition, when each plant was exposed to three adults treated with fungus, a significantly decreased transmission of TYLCV (TYTE) was observed in the infected group. However, the incidence of TYLCV -carrying MEAM1 adults (ITYAs) was not significantly different in the infected and control groups (p < 0.05). Nevertheless, a significant decrease in viral accumulation using TYLCV AC2 gene as a marker was observed in the fungus-infected MEAM1. In conclusion, the results suggested that I . fumosorosea infection decreases TYLCV accumulation in MEAM1 and subsequently reduces its transmission. Our study provides new insights into the relationship between host plant, plant virus, insect vector, and entomopathogenic fungus.