Open AccessIdentification of Yeast Mutants Exhibiting Altered Sensitivity to Valinomycin and Nigericin Demonstrate Pleiotropic Effects of Ionophores on Cellular Processes
Author(s) -
Michaela Jakúbková,
Vladimíra Džugasová,
Dominika Truban,
Lenka Abelovska,
Ingrid BhatiaKiššová,
Martin Valachovič,
Vlasta Klobučnı́ková,
Lucia Zeiselová,
Peter Griač,
Jozef Nosek,
Ľubomír Tomáška
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0164175
Subject(s) - nigericin , valinomycin , microbiology and biotechnology , saccharomyces cerevisiae , biology , biogenesis , mitochondrion , antiporter , mutant , vacuole , biochemistry , yeast , membrane potential , gene , membrane , cytoplasm
Ionophores such as valinomycin and nigericin are potent tools for studying the impact of ion perturbance on cellular functions. To obtain a broader picture about molecular components involved in mediating the effects of these drugs on yeast cells under respiratory growth conditions, we performed a screening of the haploid deletion mutant library covering the Saccharomyces cerevisiae nonessential genes. We identified nearly 130 genes whose absence leads either to resistance or to hypersensitivity to valinomycin and/or nigericin. The processes affected by their protein products range from mitochondrial functions through ribosome biogenesis and telomere maintenance to vacuolar biogenesis and stress response. Comparison of the results with independent screenings performed by our and other laboratories demonstrates that although mitochondria might represent the main target for both ionophores, cellular response to the drugs is very complex and involves an intricate network of proteins connecting mitochondria, vacuoles, and other membrane compartments.