Open AccessHomogeneous Inflammatory Gene Profiles Induced in Human Dermal Fibroblasts in Response to the Three Main Species of Borrelia burgdorferi sensu lato
Author(s) -
Mariam Meddeb,
Wassila Carpentier,
Nicolas Cagnard,
Sophie Nadaud,
Antoine Grillon,
Cathy Barthel,
Sylvie Josiane De Martino,
B. Jaulhac,
Nathalie Boulanger,
Frédéric Schramm
Publication year - 2016
Publication title -
hal (le centre pour la communication scientifique directe)
Language(s) - English
DOI - 10.1371/journal.pone.0164117.s004
Subject(s) - borrelia burgdorferi , sensu , inflammatory response , homogeneous , borrelia , microbiology and biotechnology , biology , antibody response , inflammation , immunology , antibody , zoology , physics , genus , thermodynamics
International audienceIn Lyme borreliosis, the skin is the key site for bacterial inoculation by the infected tick and for cutaneous manifestations. We previously showed that different strains of Borrelia burg-dorferi sensu stricto isolated from tick and from different clinical stages of the Lyme borrelio-sis (erythema migrans, and acrodermatitis chronica atrophicans) elicited a very similar transcriptional response in normal human dermal fibroblasts. In this study, using whole transcriptome microarray chips, we aimed to compare the transcriptional response of normal human dermal fibroblasts stimulated by 3 Borrelia burgdorferi sensu lato strains belonging to 3 main pathogenic species (B. afzelii, B. garinii and B. burgdorferi sensu stricto) in order to determine whether " species-related " inflammatory pathways could be identified. The three Borrelia strains tested exhibited similar transcriptional profiles, and no species-specific fingerprint of transcriptional changes in fibroblasts was observed. Conversely , a common core of chemokines/cytokines (CCL2, CXCL1, CXCL2, CXCL6, CXCL10, IL-6, IL-8) and interferon-related genes was stimulated by all the 3 strains. Dermal fibroblasts appear to play a key role in the cutaneous infection with Borrelia, inducing a homogeneous inflammatory response, whichever Borrelia species was involved