Open AccessA Novel Self-Assembling DNA Nano Chip for Rapid Detection of Human Papillomavirus Genes
Author(s) -
Xin Li,
Yanbo Li,
Hong Li
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0162975
Subject(s) - oligonucleotide , dna , complementary dna , chip , dna microarray , atomic force microscopy , nanotechnology , nanobiotechnology , gene , materials science , microbiology and biotechnology , gene expression , chemistry , biology , nanoparticle , computer science , biochemistry , telecommunications
Rapid detection of tumor-associated DNA such as Human Papillomavirus (HPV) has important clinical value for the early screening of tumors. By attaching oligonucleotides or cDNA onto the chip surface, DNA chip technology provides a rapid method to analyze gene expression. However, challenges remain regarding increasing probe density and improving detection time. To address these challenges, we proposed a DNA chip that was self-assembled from single stranded DNA in combination with high probe density and a rapid detection method. Over 200 probes could be attached to the surface of this 100-nm diameter DNA chip. For detection, the chips were adsorbed onto a mica surface and then incubated for ten minutes with HPV-DNA; the results were directly observable using atomic force microscopy (AFM). This bottom-up fabricated DNA nano chip combined with high probe density and direct AFM detection at the single molecule level will likely have numerous potential clinical applications for gene screening and the early diagnosis of cancer.