Open AccessSCN10A Mutation in a Patient with Erythromelalgia Enhances C-Fiber Activity Dependent Slowing
Author(s) -
Andreas M. Kist,
Sagafos Dagrun,
Anthony M. Rush,
Cristian Neacsu,
Esther Eberhardt,
Roland Schmidt,
Lars Kristian Lunden,
Kristin Ørstavik,
Kaluza Luisa,
Jannis Meents,
Zhiping Zhang,
T. Hedley Carr,
Hugh Salter,
David Malinowsky,
Patrik Wollberg,
Johannes Krupp,
Inge Petter Kleggetveit,
Martin Schmelz,
Ellen Jørum,
Angelika Lampert,
Barbara Namer
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0161789
Subject(s) - erythromelalgia , sodium channel , gating , microneurography , patch clamp , transfection , tetrodotoxin , nav1 , mutation , medicine , dorsal root ganglion , electrophysiology , neuroscience , chemistry , pharmacology , anesthesia , biology , genetics , sensory system , sodium , cell culture , gene , baroreflex , heart rate , organic chemistry , blood pressure
Gain-of-function mutations in the tetrodotoxin (TTX) sensitive voltage-gated sodium channel (Nav) Nav1.7 have been identified as a key mechanism underlying chronic pain in inherited erythromelalgia. Mutations in TTX resistant channels, such as Nav1.8 or Nav1.9, were recently connected with inherited chronic pain syndromes. Here, we investigated the effects of the p.M650K mutation in Nav1.8 in a 53 year old patient with erythromelalgia by microneurography and patch-clamp techniques. Recordings of the patient’s peripheral nerve fibers showed increased activity dependent slowing (ADS) in CMi and less spontaneous firing compared to a control group of erythromelalgia patients without Nav mutations. To evaluate the impact of the p.M650K mutation on neuronal firing and channel gating, we performed current and voltage-clamp recordings on transfected sensory neurons (DRGs) and neuroblastoma cells. The p.M650K mutation shifted steady-state fast inactivation of Nav1.8 to more hyperpolarized potentials and did not significantly alter any other tested gating behaviors. The AP half-width was significantly broader and the stimulated action potential firing rate was reduced for M650K transfected DRGs compared to WT. We discuss the potential link between enhanced steady state fast inactivation, broader action potential width and the potential physiological consequences.