Open AccessModestobacter lacusdianchii sp. nov., a Phosphate-Solubilizing Actinobacterium with Ability to Promote Microcystis Growth
Author(s) -
Bing-Huo Zhang,
Nimaichand Salam,
Juan Cheng,
Han-Quan Li,
Jin Yang,
Daiming Zha,
Yuqin Zhang,
Meng-Jie Ai,
Wael N. Hozzein,
Wenjun Li
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0161069
Subject(s) - rhamnose , arabinose , biology , 16s ribosomal rna , galactose , strain (injury) , microbiology and biotechnology , biochemistry , phosphatidylinositol , gene , xylose , fermentation , anatomy , kinase
A novel actinobacterium, designated strain JXJ CY 19 T , was isolated from a culture mat of Microcystis aeruginosa FACHB-905 collected from Dianchi Lake, South-west China. 16S rRNA gene sequences comparison of strain JXJ CY 19 T and the available sequences in the GenBank database showed that the strain was closely related to Modestobacter marinus 42H12-1 T (99.1% similarity) and Modestobacter roseus KLBMP 1279 T (99.0%). The isolate had meso -diaminopimelic in the cell wall with whole-cell sugars of mannose, rhamnose, ribose, glucose, galactose, and arabinose. The menaquinone detected was MK-9(H 4 ), while the major cellular fatty acids include C 17:1 ω 8 c , C 15:0 iso, C 15:1 iso G and C 16:0 iso. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The DNA-DNA hybridization values between strains JXJ CY 19 T and the closely related type strains Modestobacter marinus CGMCC 4.5581 T and Modestobacter roseus NBRC 108673 T were determined to be 50.8 ± 0.8% and 44.1 ± 1.7%, respectively. The DNA G+C content was 71.9 mol%. On the basis of the above taxonomic data and differences in physiological characters from the closely related type strains, strain JXJ CY 19 T was recognized as a novel species of the genus Modestobacter , for which the name Modestobacter lacusdianchii sp. nov. (JXJ CY 19 T = KCTC 39600 T = CPCC 204352 T ) is proposed. The type strain JXJ CY 19 T can solubilize calcium phosphate tribasic (Ca 3 (PO 4 ) 2 ), phytin and L - α -phosphatidylcholine. The phosphate-solubilizing property of the novel actinobacterium could be a possible factor for the increase in growth of Microcystis aeruginosa FACHB-905 in ecosystem where the amount of available soluble phosphate is limited such as Dianchi Lake.