Open AccessDifferent Biological Action of Oleic Acid in ALDHhigh and ALDHlow Subpopulations Separated from Ductal Carcinoma In Situ of Breast Cancer
Author(s) -
Hoe Suk Kim,
Minji Jung,
Sul Ki Choi,
Woo Kyung Moon,
Seung Ja Kim
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0160835
Subject(s) - biology , cancer research , ductal carcinoma , cancer , breast cancer , immunology , microbiology and biotechnology , genetics
The mechanisms underlying breast cancer progression of ductal carcinoma in situ ( DCIS) associated with fatty acids are largely unknown. In the present study, we compared the action of oleic acid (OA) on two human DCIS cell lines, MCF10DCIS.COM (ER/PR/HER2-negative) and SUM225 (HER2 overexpressed). OA led to a significant increase in proliferation, migration, lipid accumulation and the expression of lipogenic proteins, such as SREBP-1, FAS and ACC-1, in MCF10DCIS.COM cells but not SUM225 cells. The ALDH high subpopulation analyzed by the ALDEFLUOR assay was approximately 39.2±5.3% of MCF10DCIS.COM cells but was small (3.11±0.9%) in SUM225 cells. We further investigated the different biological action of OA in the distinct ALDH low and ALDH high subpopulations of MCF10DCIS.COM cells. OA led to an increase in the expression of ALDH1A1, ALDH1A2 and ALDH1A3 in MCF10DCIS.COM cells. SREBP-1 and ACC-1 were highly expressed in ALDH high cells relative to ALDH low cells, whereas FAS was higher in ALDH low cells. In the presence of OA, ALDH high cells were more likely to proliferate and migrate and displayed significantly high levels of SREBP-1 and FAS and strong phosphorylation of FAK and AKT relative to ALDH low cells. This study suggests that OA could be a critical risk factor to promote the proliferation and migration of ALDH high cells in DCIS, leading to breast cancer progression.