Open Access2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis
Author(s) -
Luděk Eyer,
Thomas Vain,
Barbora Pařízková,
Jana Oklešťková,
Elke Barbez,
Hana Kozubíková,
Tomáš Pospíšil,
Roksana Wierzbicka,
Jürgen KleineVehn,
Milan Fránek,
Miroslav Strnad,
Stéphanie Robert,
Ondřej Novák
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0159269
Subject(s) - auxin , metabolite , arabidopsis , chemistry , biochemistry , metabolism , amide , metabolic pathway , stereochemistry , mutant , gene
The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic and traceable analog to study auxin-related responses. Here we identified that not only exogenous 2,4-D but also its amide-linked metabolite 2,4-D-Glu displayed an inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate 2,4-D metabolite conversion, identity and activity, we have developed a novel purification procedure based on the combination of ion exchange and immuno-specific sorbents combined with a sensitive liquid chromatography-mass spectrometry method. In 2,4-D treated samples, 2,4-D-Glu and 2,4-D-Asp were detected at 100-fold lower concentrations compared to 2,4-D levels, showing that 2,4-D can be metabolized in the plant. Moreover, 2,4-D-Asp and 2,4-D-Glu were identified as reversible forms of 2,4-D homeostasis that can be converted to free 2,4-D. This work paves the way to new studies of auxin action in plant development.