Open AccessHnRNP Q Has a Suppressive Role in the Translation of Mouse Cryptochrome1
Author(s) -
Ilgye Lim,
Youngseob Jung,
DoYeon Kim,
KyongTai Kim
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0159018
Subject(s) - translation (biology) , messenger rna , biology , untranslated region , three prime untranslated region , circadian rhythm , gene expression , circadian clock , microbiology and biotechnology , clock , regulation of gene expression , gene , genetics , neuroscience
Precise regulation of gene expression is especially important for circadian timekeeping which is maintained by the proper oscillation of the mRNA and protein of clock genes and clock-controlled genes. As a main component of the core negative arm feedback loops in the circadian clock, the Cry1 gene contributes to the maintenance of behavioral and molecular rhythmicity. Despite the central role of Cry1, the molecular mechanisms regulating expression levels of Cry1 mRNA and protein are not well defined. In particular, the post-transcriptional regulation of Cry1 mRNA fate decisions is unclear. Here, we demonstrate that hnRNP Q binds to mCry1 mRNA via the 5′UTR. Furthermore, hnRNP Q inhibits the translation of mCry1 mRNA, leading to altered rhythmicity in the mCRY1 protein profile.