Open AccessCharacterisation of Cultured Mesothelial Cells Derived from the Murine Adult Omentum
Author(s) -
Sumaya Dauleh,
Ilaria Santeramo,
Claire Fielding,
Kelly Ward,
AnneKristin Herrmann,
Patricia Murray,
Bettina Wilm
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0158997
Subject(s) - mesothelial cell , mesenchymal stem cell , stem cell , mesothelium , microbiology and biotechnology , biology , adult stem cell , stromal cell , cellular differentiation , progenitor cell , amniotic stem cells , pathology , endothelial stem cell , in vitro , cancer research , medicine , genetics , gene
The human omentum has been long regarded as a healing patch, used by surgeons for its ability to immunomodulate, repair and vascularise injured tissues. A major component of the omentum are mesothelial cells, which display some of the characteristics of mesenchymal stem/stromal cells. For instance, lineage tracing studies have shown that mesothelial cells give rise to adipocytes and vascular smooth muscle cells, and human and rat mesothelial cells have been shown to differentiate into osteoblast- and adipocyte-like cells in vitro , indicating that they have considerable plasticity. However, so far, long-term cultures of mesothelial cells have not been successfully established due to early senescence. Here, we demonstrate that mesothelial cells isolated from the mouse omentum could be cultured for more than 30 passages. While epithelial markers were downregulated over passages in the mesothelial cells, their mesenchymal profile remained unchanged. Early passage mesothelial cells displayed clonogenicitiy, expressed several stem cell markers, and up to passage 5 and 13, respectively, could differentiate along the adipogenic and osteogenic lineages, demonstrating stem/progenitor characteristics and differentiation potential.