Open AccessEstablishment and Analysis of Cancer Stem-Like and Non-Cancer Stem-Like Clone Cells from the Human Colon Cancer Cell Line SW480
Author(s) -
Akari Takaya,
Yoshihiko Hirohashi,
Aiko Murai,
Rena Morita,
Hiroshi Shinmoto,
Eri Yamamoto,
Terufumi Kubo,
Munehide Nakatsugawa,
Takayuki Kanaseki,
Tomohide Tsukahara,
Yasuaki Tamura,
Ichiro Takemasa,
Toru Kondo,
Noriyuki Sato,
Toshihiko Torigoe
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0158903
Subject(s) - clone (java method) , cancer stem cell , biology , cd44 , cancer cell , stem cell , cell culture , population , side population , cancer research , cancer , microbiology and biotechnology , cell , genetics , medicine , gene , environmental health
Human cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) can be isolated as side population (SP) cells, aldehyde dehydrogenase high (ALDH high ) cells or cell surface marker-positive cells including CD44 + cells and CD133 + cells. CSCs/CICs and non-CSCs/CICs are unstable in in vitro culture, and CSCs/CICs can differentiate into non-CSCs/CICs and some non-CSCs/CICs can dedifferentiate into CSCs/CICs. Therefore, experiments using a large amount of CSCs/CICs are technically very difficult. In this study, we isolated single cell clones from SP cells and main population (MP) cells derived from the human colon cancer cell line SW480. SP analysis revealed that SP clone cells had relatively high percentages of SP cells, whereas MP clone cells showed very few SP cells, and the phenotypes were sustainable for more than 2 months of in vitro culture. Xenograft transplantation revealed that SP clone cells have higher tumor-initiating ability than that of MP clone cells and SP clone cell showed higher chemo-resistance compared with MP clone cells. These results indicate that SP clone cells derived from SW480 cells are enriched with CSCs/CICs, whereas MP clone cells are pure non-CSCs/CICs. SP clone cells and MP clone cells are a very stable in vitro CSC/CIC-enriched and non-CSC/CIC model for further analysis.