Open AccessThe Nuclear DNA Content and Genetic Diversity of Lampetra morii
Author(s) -
Xinyu Yan,
Weizhen Meng,
Fenfang Wu,
Anlong Xu,
Shangwu Chen,
Shengfeng Huang
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0157494
Subject(s) - lampetra , lamprey , biology , mitochondrial dna , nuclear dna , genetic diversity , genetics , phylogenetic tree , ploidy , germline , evolutionary biology , gene , population , fishery , demography , sociology
We investigated the nuclear DNA content and genetic diversity of a river lamprey, the Korean lamprey Lampetra morii , which is distributed in the northeast of China. L . morii spends its whole life cycle in fresh water, and its adult size is relatively small (~160 mm long) compared with that of other lampreys. The haploid nuclear DNA content of L . morii is 1.618 pg (approximately 1.582 Gb) in germline cells, and there is ~15% germline DNA loss in somatic cells. These values are significantly smaller than those of Petromyzon marinus , a lamprey with a published draft genome. The chromosomes of L . morii are small and acrocentric, with a diploid modal number of 2n = 132, lower than some other lampreys. Sequence and AFLP analyses suggest that the allelic polymorphism rate (~0.14% based on examined nuclear and mitochondrial DNA sequences) of L . morii is much lower than that (~2%) of P . marinus . Phylogenetic analysis based on a mitochondrial DNA fragment confirms that L . morii belongs to the genus Lampetra , which, together with the genus Lethenteron , forms a sister group to P . marinus . These genetic background data are valuable for subsequent genetic and genomic research on L . morii .