Open AccessEfficient Assessment of Developmental, Surgical and Pathological Lymphangiogenesis Using a Lymphatic Reporter Mouse and Its Embryonic Stem Cells
Author(s) -
Mingu Hong,
Eunson Jung,
Sara Yang,
Wonhyuek Jung,
Young Jin Seong,
Eunkyung Park,
Athanasios Bramos,
Kyu Eui Kim,
Sunju Lee,
George Daghlian,
Jung In Seo,
Inho Choi,
In Seon Choi,
Chester J. Koh,
Agnieszka Kobielak,
Q.Y. Ying,
Maxwell B Johnson,
Daniel J. Gardner,
Alex K. Wong,
Dongwon Choi,
Young Kwon Hong
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0157126
Subject(s) - lymphatic system , lymphangiogenesis , embryonic stem cell , lymphatic endothelium , biology , lymphatic vessel , pathology , developmental biology , stem cell , microbiology and biotechnology , medicine , cancer , genetics , metastasis , gene
Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo . In conclusion, these experimental tools and techniques will help advance lymphatic research.