Open AccessOsLBD3-7 Overexpression Induced Adaxially Rolled Leaves in Rice
Author(s) -
Cong Li,
Xiaohua Zou,
Chunyu Zhang,
Qinghao Shao,
Jun Li,
Bin Liu,
Hongyu Li,
Tao Zhao
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0156413
Subject(s) - biology , gene , transcription factor , microbiology and biotechnology , activator (genetics) , transgene , botany , genetics
Appropriate leaf rolling enhances erect-leaf habits and photosynthetic efficiency, which consequently improves grain yield. Here, we reported the novel lateral organ boundaries domain ( LBD ) gene OsLBD3-7 , which is involved in the regulation of leaf rolling. OsLBD3-7 works as a transcription activator and its protein is located on the plasma membrane and in the nucleus. Overexpression of OsLBD3-7 leads to narrow and adaxially rolled leaves. Microscopy of flag leaf cross-sections indicated that overexpression of OsLBD3-7 led to a decrease in both bulliform cell size and number. Transcriptional analysis showed that key genes that had been reported to be negative regulators of bulliform cell development were up-regulated in transgenic plants. These results indicated that OsLBD3-7 might acts as an upstream regulatory gene of bulliform cell development to regulate leaf rolling, which will give more insights on the leaf rolling regulation mechanism.