Open AccessHighly Sensitive Detection of Melamine Using a One-Step Sample Treatment Combined with a Portable Ag Nanostructure Array SERS Sensor
Author(s) -
Jie Cheng,
Xiaoou Su,
Yue Yao,
Caiqin Han,
Shi Wang,
Yiping Zhao
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0154402
Subject(s) - melamine , raman spectroscopy , nanorod , materials science , detection limit , analytical chemistry (journal) , solvent , extraction (chemistry) , methanol , nanotechnology , chromatography , chemistry , optics , composite material , physics , organic chemistry
There is an urgent need for rapid and reliable methods able to detect melamine in animal feed. In this study, a quick, simple, and sensitive method for the determination of melamine content in animal feed was developed using surface-enhanced Raman spectroscopy on fabricated Ag nanorod (AgNR) array substrates with a one-step sample extraction procedure. The AgNR array substrates washed by HNO 3 solvent (10 −7 M) and methanol and showed the good stability within 6 months. The Raman shift at △ ν = 682 cm −1 was used as the characteristic melamine peak in the calculations. Sufficient linearity was obtained in the 2–200 μg·g −1 range ( R 2 = 0.926). The limits of detection and quantification were 0.9 and 2 μg·g −1 , respectively. The recovery rates were 89.7–93.3%, with coefficients of variation below 2.02%. The method showed good accuracy compared with the tradition GC-MS analysis. This new protocol only need 2 min to fininsh the detection which could be developed for rapid onsite screening of melamine contamination in quality control and market surveillance applications.