Open AccessCharacterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance
Author(s) -
Xingpeng Duan,
Zhidong Zhang,
Jin Wang,
Kaijing Zuo
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0153988
Subject(s) - verticillium dahliae , verticillium wilt , biology , jasmonic acid , prohibitin , verticillium , effector , gene silencing , plant disease resistance , gene expression , microbiology and biotechnology , gene , genetics , botany
Verticillium wilt is a disastrous vascular disease in plants caused by Verticillium dahliae . Verticillium pathogens secrete various disease-causing effectors in cotton. This study identified a subtilase gene GbSBT1 from Gossypium babardense and investigated the roles against V . dahliae infection. GbSBT1 gene expression is responsive to V . dahliae defense signals, jasmonic acid, and ethylene treatments. Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments. Silencing GbSBT1 gene expression through virus-induced GbSBT1 gene silencing reduced the tolerance of Pima-90 (resistant genotype), but not facilitated the infection process of V . dahliae in Coker-312 (sensitive genotype). Moreover, the ectopically expressed GbSBT1 gene enhanced the resistance of Arabidopsis to Fusarium oxysporum and V . dahliae infection and activated the expression levels of defense-related genes. Furthermore, pull-down, yeast two-hybrid assay, and BiFC analysis revealed that GbSBT1 interacts with a prohibitin (PHB)-like protein expressed in V . dahliae pathogens during infection. In summary, GbSBT1 recognizes the effector PHB protein secreted from V . dahliae and is involved in Verticillium -induced resistance in cotton.