Open AccessShort-Fragment DNA Residue from Vaccine Purification Processes Promotes Immune Response to the New Inactivated EV71 Vaccine by Upregulating TLR9 mRNA
Author(s) -
Jiang Shao,
Fan Gao,
Huijuan Lin,
Qunying Mao,
Pan Chen,
Xing Wu,
Xin Yao,
Wei Kong,
Zhenglun Liang
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0153867
Subject(s) - dna vaccination , dna , nuclease , immune system , biology , tlr9 , virology , vero cell , microbiology and biotechnology , genomic dna , chemistry , gene , gene expression , virus , genetics , immunization , dna methylation
To reduce potential oncogenic long genomic DNA in vaccines, nuclease treatment has been applied in the purification processes. However, this action increased the residue of short-fragment DNA and its effect on vaccine potency was still elusive. In this study, we found residual sf-DNA in an inactivated EV71 vaccine could enhance humoral immune response in mice. Ag stimulation in vitro and vaccine injection in vivo revealed that TLR9 transcription level was elevated, indicating that sf-DNA could activate TLR9. These new findings will help us to understand the molecular mechanism induced by vero-cell culture-derived vaccines.