Open AccessDevelopment of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus
Author(s) -
Sarah V. Maifeld,
Ro Bodrey,
Hoyin Mok,
Chu Marla,
Li Yu,
Yamagata Ryan,
Leonardson Tansy,
Chio Vera,
Bandita Parhy,
Samuel Park,
Carlson Marcia,
Shushil Machhi,
Nancy D. Ulbrandt,
Ann R. Falsey,
Edward E. Walsh,
C. Kathy Wang,
Mark T. Esser,
Fengrong Zuo
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0153019
Subject(s) - serology , concordance , virology , antigen , gold standard (test) , medicine , immunology , virus , population , antibody , environmental health
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized “gold standard” panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the “gold standard” diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population.