Open AccessA SNP-Based Molecular Barcode for Characterization of Common Wheat
Author(s) -
Lifeng Gao,
Jizeng Jia,
Xiuying Kong
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0150947
Subject(s) - germplasm , snp genotyping , biology , genotyping , single nucleotide polymorphism , barcode , snp , molecular inversion probe , microbiology and biotechnology , cultivar , genetic diversity , snp array , genetics , agronomy , genotype , gene , medicine , population , computer science , environmental health , operating system
Wheat is grown as a staple crop worldwide. It is important to develop an effective genotyping tool for this cereal grain both to identify germplasm diversity and to protect the rights of breeders. Single-nucleotide polymorphism (SNP) genotyping provides a means for developing a practical, rapid, inexpensive and high-throughput assay. Here, we investigated SNPs as robust markers of genetic variation for typing wheat cultivars. We identified SNPs from an array of 9000 across a collection of 429 well-known wheat cultivars grown in China, of which 43 SNP markers with high minor allele frequency and variations discriminated the selected wheat varieties and their wild ancestors. This SNP-based barcode will allow for the rapid and precise identification of wheat germplasm resources and newly released varieties and will further assist in the wheat breeding program.