Open AccessAtGRP3 Is Implicated in Root Size and Aluminum Response Pathways in Arabidopsis
Author(s) -
Amanda Mangeon,
Renan Pardal,
Adriana Dias Menezes-Salgueiro,
Guilherme Coutinho Kullmann Duarte,
Ricardo de Seixas,
Fernanda Pérez Cruz,
Vanessa Cardeal,
Cláudia Magioli,
Felipe Klein Ricachenevsky,
Rogério Margis,
Gilberto Sachetto-Martins
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0150583
Subject(s) - arabidopsis , arabidopsis thaliana , biology , microbiology and biotechnology , mutant , transgene , phenotype , elongation , root hair , genetics , botany , gene , materials science , metallurgy , ultimate tensile strength
AtGRP3 is a glycine-rich protein (GRP) from Arabidopsis thaliana shown to interact with the receptor-like kinase AtWAK1 in yeast, in vitro and in planta . In this work, phenotypic analyses using transgenic plants were performed in order to better characterize this GRP. Plants of two independent knockout alleles of AtGRP3 develop longer roots suggesting its involvement in root size determination. Confocal microscopy analysis showed an abnormal cell division and elongation in grp3-1 knockout mutants. Moreover, we also show that grp3-1 exhibits an enhanced Aluminum (Al) tolerance, a feature also described in AtWAK1 overexpressing plants. Together, these results implicate AtGRP3 function root size determination during development and in Al stress.