Open AccessSuper-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations
Author(s) -
Michael J. Mlodzianoski,
Nikki M. Curthoys,
Mudalige S. Gunewardene,
Sean Carter,
Samuel T. Hess
Publication year - 2016
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0147506
Subject(s) - microscopy , resolution (logic) , molecule , spectral line , emission spectrum , fluorescence , fluorescence microscope , photoactivated localization microscopy , nanoscopic scale , spectral imaging , spectral resolution , biophysics , physics , materials science , optics , nuclear magnetic resonance , super resolution microscopy , nanotechnology , biology , computer science , artificial intelligence , quantum mechanics , astronomy
Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.