Open AccessExpression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis
Author(s) -
Xiangrong Dong,
Bing Teng,
Shang Dai,
Tao Li,
Linna Guo,
Zhongfang Tan,
Jiao Zhang,
Qingsheng Jin,
Yanping Wang,
Yuejin Hua
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0142918
Subject(s) - lactococcus lactis , deinococcus radiodurans , lactic acid , bacteria , streptococcaceae , biology , strain (injury) , chemistry , microbiology and biotechnology , biochemistry , genetics , anatomy
PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans . In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L . lactis while under stress. PprI was stably expressed in L . lactis as confirmed by western blot assays. L . lactis expressing PprI exhibited significantly improved resistance to oxidative stress and high osmotic pressure. This enhanced cellular tolerance to stressors might be due to the regulation of resistance-related genes (e.g., recA , recO , sodA , and nah ) by pprI . Moreover, transformed L . lactis demonstrated increased lactic acid production, attributed to enhanced lactate dehydrogenase activity. These results suggest that pprI can improve the tolerance of L . lactis to environmental stresses, and this transformed bacterial strain is a promising candidate for industrial applications of lactic acid production.