Open AccessThe Analysis of a Microbial Community in the UV/O3-Anaerobic/Aerobic Integrated Process for Petrochemical Nanofiltration Concentrate (NFC) Treatment by 454-Pyrosequencing
Author(s) -
Chao Wei,
Wei He,
Wei Li,
Chunying Li,
Jun Ma
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0139991
Subject(s) - nitrospira , biodegradation , environmental chemistry , chemistry , wastewater , activated sludge , stenotrophomonas , chemical oxygen demand , microbial consortium , microbial population biology , biochemical oxygen demand , nitrite , microbiology and biotechnology , bacteria , microorganism , biology , pseudomonas , organic chemistry , environmental engineering , nitrate , environmental science , genetics
In this study, high-throughput pyrosequencing was applied on the analysis of the microbial community of activated sludge and biofilm in a lab-scale UV/O 3 - anaerobic/aerobic (A/O) integrated process for the treatment of petrochemical nanofiltration concentrate (NFC) wastewater. NFC is a type of saline wastewater with low biodegradability. From the anaerobic activated sludge (Sample A) and aerobic biofilm (Sample O), 59,748 and 51,231 valid sequence reads were obtained, respectively. The dominant phylotypes related to the metabolism of organic compounds, polycyclic aromatic hydrocarbon (PAH) biodegradation, assimilation of carbon from benzene, and the biodegradation of nitrogenous organic compounds were detected as genus Clostridium , genera Pseudomonas and Stenotrophomonas , class Betaproteobacteria , and genus Hyphomicrobium . Furthermore, the nitrite-oxidising bacteria Nitrospira , nitrite-reducing and sulphate-oxidising bacteria (NR-SRB) Thioalkalivibrio were also detected. In the last twenty operational days, the total Chemical Oxygen Demand (COD) and Total Organic Carbon (TOC) removal efficiencies on average were 64.93% and 62.06%, respectively. The removal efficiencies of ammonia nitrogen and Total Nitrogen (TN) on average were 90.51% and 75.11% during the entire treatment process.