Open AccessFree and Cell Wall-Bound Polyamines under Long-Term Water Stress Applied at Different Growth Stages of ×Triticosecale Wittm
Author(s) -
Tomasz Hura,
Michał Dziurka,
Katarzyna Hura,
Agnieszka Ostrowska,
Kinga Dziurka
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0135002
Subject(s) - spermidine , putrescine , spermine , agmatine , polyamine , cadaverine , anthesis , cultivar , biology , cell wall , triticale , botany , horticulture , biochemistry , enzyme
Background Long-stemmed and semi-dwarf cultivars of triticale were exposed to water stress at tillering, heading and anthesis stage. Quantitative determination of free and cell wall-bound polyamines, i.e. agmatine, cadaverine, putrescine, spermidine and spermine, was supplemented with an analysis of quantitative relationships between free and cell wall-bound polyamines. Results The content of free and cell wall-bound polyamines varied depending on the development stage, both under optimal and water stress conditions. Drought-induced increase in free agmatine content was observed at all developmental stages in long-stemmed cultivar. A depletion of spermidine and putrescine was also reported in this cultivar, and spermidine was less abundant in semi-dwarf cultivar exposed to drought stress at the three analyzed developmental stages. Changes in the content of the other free polyamines did not follow a steady pattern reflecting the developmental stages. On the contrary, the content of cell wall-bound polyamines gradually increased from tillering, through heading and until anthesis period. Conclusion Water stress seemed to induce a progressive decrease in the content of free polyamines and an accumulation of cell wall-bound polyamines.