Open AccessCytoprotective Effects of Grape Seed Extract on Human Gingival Fibroblasts in Relation to Its Antioxidant Potential
Author(s) -
Yusuke Katsuda,
Yoshimi Niwano,
Takuji Nakashima,
Takayuki Mokudai,
Keisuke Nakamura,
Satoshi Oizumi,
Taro Kanno,
Hiroyasu Kanetaka,
Hiroshi Egusa
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0134704
Subject(s) - trolox , antioxidant , reactive oxygen species , chemistry , hydrogen peroxide , grape seed extract , dpph , oxidative stress , singlet oxygen , biochemistry , intracellular , hydroxyl radical , food science , oxygen , medicine , organic chemistry , alternative medicine , pathology
Cytoprotective effects of short-term treatment with grape seed extract (GSE) upon human gingival fibroblasts (hGFs) were evaluated in relation to its antioxidant properties and compared with those of a water-soluble analog of vitamin E: trolox (Tx). GSE and Tx showed comparable antioxidant potential in vitro against di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH; a stable radical), hydroxyl radical ( • OH), singlet oxygen ( 1 O 2 ), and hydrogen peroxide (H 2 O 2 ). Pretreatment or concomitant treatment with GSE for 1 min protected hGFs from oxidative stressors, including H 2 O 2 , acid-electrolyzed water (AEW), and 1 O 2 , and attenuated the intracellular formation of reactive oxygen species induced by H 2 O 2 and AEW. Tx also reduced the H 2 O 2 - and AEW-induced intracellular formation of reactive oxygen species, but showed no cytoprotective effects on hGFs exposed to H 2 O 2 , AEW, or 1 O 2 . These results suggest that the cytoprotective effects of GSE are likely exerted independently of its antioxidant potential.