Open AccessCharacterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1
Author(s) -
Yang Cheng,
Feng Lu,
Seong-Kyun Lee,
Deok-Hoon Kong,
KwonSoo Ha,
Bo Wang,
Jetsumon Sattabongkot,
Takafumi Tsuboi,
EunTaek Han
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0127500
Subject(s) - biology , intracellular , plasmodium vivax , intracellular parasite , antigenicity , membrane protein , organelle , antibody , parasite hosting , red blood cell , virology , plasmodium (life cycle) , apical membrane , recombinant dna , microbiology and biotechnology , biochemistry , plasmodium falciparum , membrane , immunology , malaria , gene , world wide web , computer science
In Plasmodium , the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of Plasmodium species have been identified such as early-transcribed membrane proteins (ETRAMPs) and exported proteins (EXPs). However, there is little or no information regarding the intracellular parasite membrane in Plasmodium vivax . In the present study, recombinant PvETRAMP11.2 (PVX_003565) and PvEXP1 (PVX_091700) were expressed and evaluated antigenicity tests using sera from P . vivax -infected patients. A large proportion of infected individuals presented with IgG antibody responses against PvETRAMP11.2 (76.8%) and PvEXP1 (69.6%). Both of the recombinant proteins elicited high antibody titers capable of recognizing parasites of vivax malaria patients. PvETRAMP11.2 partially co-localized with PvEXP1 on the intracellular membranes of immature schizont. Moreover, they were also detected at the apical organelles of newly formed merozoites of mature schizont. We first proposed that these proteins might be synthesized in the preceding schizont stage, localized on the parasite membranes and apical organelles of infected erythrocytes, and induced high IgG antibody responses in patients.