Open AccessOrganelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling
Author(s) -
Jianhua Wang,
Yuzheng Zhao,
Chao Wang,
Qian Zhu,
Zengmin Du,
Aiguo Hu,
Yi Yang
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0123986
Subject(s) - organelle , nitric oxide , microbiology and biotechnology , chemistry , function (biology) , biophysics , nanotechnology , biology , materials science , organic chemistry
Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo . NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.