Open AccessThe Initial Inflammatory Response to Bioactive Implants Is Characterized by NETosis
Author(s) -
Ljubomir Vitkov,
WolfDietrich Krautgartner,
Astrid Obermayer,
Walter Stoiber,
Matthias Hannig,
Michaela Klappacher,
Dominik Hartl
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0121359
Subject(s) - neutrophil extracellular traps , osseointegration , inflammation , citrullination , confocal , chemistry , microbiology and biotechnology , biophysics , confocal microscopy , inflammatory response , citrulline , biomedical engineering , materials science , biochemistry , immunology , biology , medicine , implant , arginine , amino acid , surgery , geometry , mathematics
Implants trigger an inflammatory response, which is important for osseointegration. Here we studied neutrophil extracellular trap (NET) release of human neutrophils in response to sandblasted large-grit acid etched (SLA) implants using fluorescent, confocal laser scanning and scanning electron microscopy. Our studies demonstrate that human neutrophils rapidly adhered to SLA surfaces, which triggered histone citrullination and NET release. Further studies showed that albumin or acetylsalicylic acid had no significant effects on the inflammatory response to SLA surfaces. In contrast to bioinert materials, which do not osseointegrate, the bioactivity of SLA surfaces is coupled with the ability to release NETs. Further investigations are necessary for clarifying the role of NETosis for osseointegration.