Open AccessSeparation of Folinic Acid Diastereomers in Capillary Electrophoresis Using a New Cationic β-Cyclodextrin Derivative
Author(s) -
Jia Yu,
Xinlei Liang,
Zhaokun Wang,
Xin Guo,
Tiemin Sun,
Xingjie Guo
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0120216
Subject(s) - capillary electrophoresis , diastereomer , cyclodextrin , chemistry , folinic acid , chromatography , cationic polymerization , acetonitrile , electrophoresis , organic chemistry , medicine , colorectal cancer , cancer
A method for the separation of folinic acid diastereomers by capillary electrophoresis in chiral separation media was developed. Aiming to achieve a good separation of the anionic analytes, a newly synthesized cationic β -cyclodextrin derivative, mono-6-deoxy-6-piperdine- β -cyclodextrin, was applied as the chiral selector. The effect of background electrolyte pH, the concentration of the cyclodextrin additive, and organic modifier on the separation was investigated. A good separation of folinic acid diastereomers was obtained with 30 mmol/L phosphate buffer at pH 6.50 containing 6.0 mmol/L of mono-6-deoxy-6-piperdine- β -cyclodextrin in 10% acetonitrile. Based on the capillary electrophoresis data, the binding constants of each diastereomer with mono-6-deoxy-6-piperdine- β -cyclodextrin were determined. Moreover, a computational modeling study, using the semi-empirical PM3 method, was used to discuss the possible mechanism of separation of folinic acid with mono-6-deoxy-6-piperdine- β -cyclodextrin.