Open AccessProteomics-Driven Analysis of Ovine Whey Colostrum
Author(s) -
Domenica Scumaci,
Francesca Trimboli,
Ludovica Dell’Aquila,
Antonio Concolino,
Giusi Pappaianni,
Laura Tammè,
G. Vignola,
A Luciani,
Daniela Morelli,
Giovanni Cuda,
Andrea Boari,
Domenico Britti
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0117433
Subject(s) - colostrum , proteome , proteomics , biology , trypsin , antibody , biochemistry , immunology , enzyme , gene
The aim of this study was to shed light in to the complexity of the ovine colostrum proteome, with a specific focus on the low abundance proteins. The ovine colostrum is characterized by a few dominating proteins, as the immunoglobulins, but it also contains less represented protein species, equally important for the correct development of neonates. Ovine colostrum, collected immediately after lambing, was separated by 1D SDS-PAGE. Proteins bands were digested with trypsin and the resulting peptides were analyzed by LC-MS/MS. On the basis of the Swiss-Prot database, a total of 343 unique proteins were identified. To our knowledge, this study represents the most comprehensive analysis of ovine colostrum proteome.