Open AccessMetabolism of Skin-Absorbed Resveratrol into Its Glucuronized Form in Mouse Skin
Author(s) -
Ichiro Murakami,
Romanas Chaleckis,
Tomáš Pluskal,
Ken Ito,
Kousuke Hori,
Masahiro Ebe,
Mitsuhiro Yanagida,
Eiji Kondoh
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0115359
Subject(s) - resveratrol , bioavailability , oral administration , chemistry , absorption (acoustics) , inflammation , pharmacology , metabolism , biochemistry , biology , medicine , physics , acoustics
Resveratrol (RESV) is a plant polyphenol, which is thought to have beneficial metabolic effects in laboratory animals as well as in humans. Following oral administration, RESV is immediately catabolized, resulting in low bioavailability. This study compared RESV metabolites and their tissue distribution after oral uptake and skin absorption. Metabolomic analysis of various mouse tissues revealed that RESV can be absorbed and metabolized through skin. We detected sulfated and glucuronidated RESV metabolites, as well as dihydroresveratrol. These metabolites are thought to have lower pharmacological activity than RESV. Similar quantities of most RESV metabolites were observed 4 h after oral or skin administration, except that glucuronidated RESV metabolites were more abundant in skin after topical RESV application than after oral administration. This result is consistent with our finding of glucuronidated RESV metabolites in cultured skin cells. RESV applied to mouse ears significantly suppressed inflammation in the TPA inflammation model. The skin absorption route could be a complementary, potent way to achieve therapeutic effects with RESV.