Open AccessSub1 and Maf1, Two Effectors of RNA Polymerase III, Are Involved in the Yeast Quiescence Cycle
Author(s) -
Joël Acker,
Ngoc-Thuy-Trinh Nguyen,
Marie Vandamme,
Arounie Tavenet,
Audrey Briand-Suleau,
Christine Conesa
Publication year - 2014
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0114587
Subject(s) - transcription (linguistics) , rna polymerase iii , microbiology and biotechnology , rna polymerase ii , effector , biology , saccharomyces cerevisiae , rna polymerase , yeast , genetics , rna , gene , gene expression , promoter , philosophy , linguistics
Sub1 and Maf1 exert an opposite effect on RNA polymerase III transcription interfering with different steps of the transcription cycle. In this study, we present evidence that Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span. First, cells lacking Sub1 need more time than wild type to exit from resting and this lag in re-proliferation is correlated with a delay in transcriptional reactivation. Second, our data show that the capacity of the cells to properly establish a quiescent state is impaired in the absence of Sub1 resulting in a premature death that is dependent on the Ras/PKA and Tor1/Sch9 signalling pathways. On the other hand, we show that maf1Δ cells are long-lived mutant suggesting a connection between Pol III transcription and yeast longevity.